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American Society of Microbiology ABMM Sample Questions:

1. A research laboratory is investigating the mechanisms of antiviral drug resistance. They are studying a herpes simplex virus strain that has developed resistance to acyclovir. The MOST common mechanism of acyclovir resistance in HSV involves mutations in the gene encoding:

A) Viral glycoprotein D
B) Viral DNA polymerase
C) Viral protease
D) Viral thymidine kinase

2. A clinical microbiology laboratory is using a nucleic acid amplification test (NAAT) to detect Mycobacterium tuberculosis directly from sputum. A positive result indicates:

A) Drug susceptibility information for the M. tuberculosis strain.
B) Active tuberculosis disease requiring immediate treatment.
C) The presence of viable M. tuberculosis organisms.
D) The presence of M. tuberculosis DNA, which could be from viable or non-viable organisms.

3. A research laboratory is investigating the mechanism of action of a novel antiviral compound. In vitro studies demonstrate that the compound effectively inhibits the replication of a specific enveloped RNA virus. Further analysis reveals that the compound does not interfere with viral entry, genome replication, or protein synthesis. However, electron microscopy of infected cells treated with the compound shows an accumulation of viral glycoproteins within the endoplasmic reticulum and Golgi apparatus, with a significant reduction in the release of infectious virions. The MOST likely target of this antiviral compound is:

A) Host cell membrane fusion machinery
B) Viral RNA-dependent RNA polymerase
C) Viral protease responsible for polyprotein cleavage
D) Viral glycoprotein trafficking and maturation

4. A patient with a history of heart valve replacement develops subacute bacterial endocarditis. Blood cultures grow a Gram-positive coccus that is alpha-hemolytic on blood agar, catalase-negative, and optochin-resistant.
The MOST likely organism is:

A) Streptococcus pneumoniae
B) Streptococcus pyogenes
C) Viridans streptococci
D) Enterococcus faecalis

5. A clinical microbiology laboratory is performing antimicrobial susceptibility testing on a Streptococcus pneumoniae isolate using broth microdilution. The lowest concentration of antibiotic that inhibits visible growth of the organism is reported as the:

A) Minimum bactericidal concentration (MBC)
B) Zone diameter
C) Breakpoint
D) Minimum inhibitory concentration (MIC)

Solutions: